Grant: $499,307 - National Institutes of Health - Sep. 21, 2009
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Award Description: This project directly addressed the broad Challenge Area (06) Enabling Technologies and specific Challenge Topic: Chemist/Biologist collaborations facilitating tool development. The goal of this project is to target the N?linked glycans on ion-conducting and regulatory subunits of K+ channels found in cardiomyocytes. There are three aims of this project. In Aim 1, the Kobertz laboratory will chemically-derivatize peptide toxins such that when they bind to their cognate K+ channel complex they will covalently label the N-linked glycans on the proteins in the complex with a chemical probe. The labeling efficiency and specificity of the chemical reactions will be determined in standard laboratory cell lines. Aims 2 and 3 (Colecraft Laboratory, Columbia Medical School) will demonstrate the utility of the panel of derivatized toxins by identifying the native regulatory subunits co?assembled with cardiac K+ channels and determining the cell surface localization and turnover of K+ channels in living cardiomyocytes.
Project Description: None to date Award starts on 9/30/09
Jobs Summary: N/A (Total jobs reported: 0)
Project Status: Not Started
This award's data was last updated on Sep. 21, 2009. Help expand these official descriptions using the wiki below.