UNIVERSITY OF TOLEDO, THE
Grant: $293,721 - National Institutes of Health - Jul. 15, 2009
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Award Description: Goals: Our initial goal is to define the molecular pathways that regulate RKIP expression in prostate cancer. The long-term goal of this research is to better define the role of RKIP as a prostate cancer metastasis suppressor in vivo, and to identify molecular targets that can be manipulated therapeutically to affect the RKIP expression in prostate cancer.Proposed Methods and Specific Aims Specific Aim #1: Elucidate the transcriptional regulation of prostate cancer RKIP expression In order to identify the regulators of RKIP expression in human prostate cancer cells, we will study two transcription factors – Snail and EZH2 – which we have identified as putative regulators of RKIP expression. The roles of these candidate regulators will be defined by a combination of loss-of-function and gain-of-function approaches. Next, we will identify the cis-elements on the RKIP promoter that are recognized by the studied regulatory elements, and determine whether these putative regulators bind directly to the promoter. Specific Aim #2: Examine the relationships between expression of RKIP and its candidate TFs in prostate cancer samples Human prostate cancer tissue will be used to determine the relationship between the expression patterns of the two putative regulators to RKIP in morphologically normal versus prostate cancer tissues. This will be done by immunohistochemical (IHC) staining of human tissue microarrays (TMA) and analysis of published DNA microarray datasets.
Project Description: We have succeeded in identifying the cis-elements on RKIP promoter that are recognized by Snail and EZH2 transription factors. The identified elements are essential for the regulation of RKIP expression in prostate cancer.
Jobs Summary: One full time and two part time jobs have been created. A Research Associate was hired to contribute 6 calendar months towards this project annually. This position requires a master's degree in Biology.The hired Research Associate is in charge of the microinjection and tissue culture facilities of the laboratory. She has expertise in all the basic molecular biology techniques. She will be heavily involved in the hand-on aspects of the various experiments of this proposal. One full time and one part time (50%) Research Assistants were hired to assist the Research Associate in the execution of the various experiments of this proposal. The two newly hired Research Assistants were fresh college graduates and the newly hired Reseach Associate was unemployed prior to this job. (Total jobs reported: 2)
Project Status: Less Than 50% Completed
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