Grant: $194,838 - National Institutes of Health - Jul. 17, 2009
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Award Description: The long term objective of this proposal is to address the role of IFN??signaling in the regulation of CD4 T cell responses during Mtb infection. Using a well-established mouse model of tuberculosis we will test the hypothesis that IFN??acts directly on CD4 T cells to regulate their differentiation, function and turnover during Mtb infection. We will compare Mtb-specific CD4 T cells that do not express a functional IFN??receptor, which are therefore are unable to respond to IFN??to wild type Mtb-specific CD4 T cells. This approach will allow us to address the direct effects of IFN??on CD4 T cells in an otherwise normal host environment (i.e., all other cell types express a functional IFN??receptor). In Aim 1 we will analyze expansion and activation of wild type and IFN??receptor-deficient Mtb-specific CD4 T cells in the lungs and relevant lymphoid tissues of Mtb infected mice. Aim 2 will address whether IFN??promotes contraction and/or differentiation of CD4 T cells during Mtb infection. PUBLIC HEALTH RELEVANCE. Tuberculosis is a major public health threat of worldwide proportions. The proposed studies will provide information essential for understanding the genesis of protective CD4 T cell responses during tuberculosis infections, so the data generated will be generally applicable to the development of vaccines and therapies designed to elicit CD4 T cell-mediated immunity in humans
Project Description: During the first phase of the project we have initiated the mouse breeding studies in order to generate a T cell receptor transgenic mouse strain that lacks the IFNGR
Jobs Summary: Trudeau Institute has retained a senior post-doctoral associate to undertake these studies. Dr. William Reiley, Ph.D. (50% effort) will be responsible for performing all of the studies that use infected mice. He will participate in the design and execution of the experiments, the interpretations of the results, and writing the manuscripts. Lab management, animal husbandry, tissue culture, immunology and infectious disease research. new hire (Total jobs reported: 0)
Project Status: Less Than 50% Completed
This award's data was last updated on Jul. 17, 2009. Help expand these official descriptions using the wiki below.
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