Grant: $11,556 - National Institutes of Health - Jul. 21, 2009
0% voted satisfied - 100% voted not satisfied - 1 vote(s) cast
Award Description: If successful these studies may allow us to use foamy EF1alpha vectors to express canine pyruvate kinase in the Basenji dog autologous transplant model as part of the parent proposal. These studies may also provide data and reagents that can be used for a wide variety of gene transfer applications including erythroid diseases. The supplement also provides a highly motivated undergraduate student opportunities to have a research project and enhance a career in the biomedical sciences. The proposed work thus directly addresses the Recovery Act goal to encourage students to seriously pursue research careers in the health related sciences by providing a short term research experience in an NIH-funded laboratory.
Project Description: Foamy vectors with an EF1alpha promoter were constructed and prepared. Transgene expression was analyzed. The student gained many research skills and learned how to execute a cloning strategy following different approaches in order to construct a foamy vector with inserts from different sources, plasmid and 293T cells during this lab experience period. This involved DNA preparation from bulk bacterial colonies to be used for cloning purposes and from clonal populations for molecular analysis. The student also had to PCR desired fragments from plasmid and genomic DNA templates and use these amplified fragments for cloning. The student was educated in multiple lab techniques aside from cloning as well.
Jobs Summary: To support an undergraduate college student to conduct a short term laboratory research project and to encourage a career in the biomedical sciences. The research experience includes training in laboratory techniques and procedures and an opportunity to conduct a project in a NIH-funded laboratory. (Total jobs reported: 1)
Project Status: More than 50% Completed
This award's data was last updated on Jul. 21, 2009. Help expand these official descriptions using the wiki below.