University of Pittsburgh
Grant: $117,503 - National Institutes of Health - Sep. 1, 2009
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Award Description: The objective of this request is to more quickly achieve the goals of our NIDDK-funded research into 'Adenosine Receptor Function in Bladder Uroepithelium.' The purpose of this parent grant is to test the hypothesis that adenosine, acting through uroepithelial-associated adenosine receptors, stimulates membrane turnover in the umbrella cell layer and alters bladder function. The results of these studies will increase our understanding of the role of adenosine and its receptors in the uroepithelium and bladder function, and will ultimately allow us to understand how perturbations in uroepithelial-associated receptor expression and/or release of mediators can contribute to bladder diseases such as interstitial cystitis and detrusor over activity. Many of our proposed studies employ transmission electron microscopy (TEM) and scanning EM, which are labor intensive and require a multi-step process to prepare samples prior to viewing them in the electron microscope. The aim of the requested supplement is to recruit a research technician with skills in electron microscopy that will accelerate the pace of our research program.
Project Description: We requested an administrative supplement utilizing recovery act funds to more quickly achieve the goals of our NIDDK-funded research into 'Adenosine Receptor Function in Bladder Uroepithelium.' At present little is understood about the mechanisms of adenosine biosynthesis in the uroepithelium or the sensory role of adenosine in modulating normal uroepithelial functions such as exocytosis/endocytosis and bladder filling/voiding. Our studies thus far indicate that adenosine stimulates membrane turnover in the uroepithelium, and when it is instilled into the bladder it slows detrusor function. The goal of the parent grant is to test the hypothesis that adenosine, acting through uroepithelial-associated adenosine receptors, functions as a sensory input molecule that stimulates membrane turnover in the umbrella cell layer and alters bladder function. The results of these studies will increase our understanding of the role of adenosine and its receptors in the uroepithelial-associated sensory web, and will ultimately allow us to understand how perturbations in uroepithelial-associated receptor expression and/or release of mediators can contribute to bladder diseases such as interstitial cystitis and detrusor overactivity. At present our research efforts are slowed by the laborious nature of performing the animal experiments and preparing samples for electron microscopy. The supplement had one aim, which was to fund an additional research position to accelerate our research progress. We have filled the position.
Jobs Summary: Prime Recipient created a Graduate Student Researcher position. (Total jobs reported: 1)
Project Status: Less Than 50% Completed
This award's data was last updated on Sep. 1, 2009. Help expand these official descriptions using the wiki below.
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