Grant: $145,825 - National Institutes of Health - Sep. 11, 2009
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Award Description: In this competive renewal period we will: 1) Continue to define function of PfCRT isoforms via heterologous expression in yeast and analysis of purified membrane, ISOV, and PL preparations. We will use recently invented techniques and chemical probes for drug, amino acid, and ion binding and transport. We will alsosynthesize additional probes (e.g. AzB-MQ, AzBCQ side chain length variants, AzB-QN) for PfCRT function. These probes will also be used in Aim 3. 2) Continue to defme drug transport functions of PfCRT isoforms using ISOV and PLs and radio labeled and fluorescent (e.g. NBD - CQ) probes. We will also synthesize additional probes (e.g. NBD - MQ, NBD - QN) using previously synthesized intermediates and similar chemistry relative to successful synthesis ofNBD - CQ. 3) Test hypotheses for function ofPfMDRl following a similar approach, and also using high throughput plate based ATPase assays we have developed. We will investigate the unusual (relative to other ABCB transporters) drug - influenced 'communication' between the two symmetrical halves ofPfMDRl. We will also analyze binding, transport and ATPase properties ofISOVs and PLs harboring known ratios of various PfCRT and PfMDRl proteins to test for interaction between the two transporters. '
Project Description: The function of antimalarial drug resistance proteins
Jobs Summary: None (Total jobs reported: 0)
Project Status: Not Started
This award's data was last updated on Sep. 11, 2009. Help expand these official descriptions using the wiki below.