Grant: $164,290 - National Institutes of Health - Aug. 28, 2009
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Award Description: This supplement will enhance and accelerate the research described in the project R01 GM082843-01A2 by taking advantage of new technologies and sources of data. The goal of the parent project is to identify the molecular pathways responsible for the origin and diversification of the 'sex comb – a novel male-specific morphological structure in Drosophila. Towards that goal, genome-wide transcriptome analysis will be used to compare gene expression between males and females of Drosophila species that display divergent and convergent sex comb morphologies. Supplemental funding will be used to implement new techniques that will enable more sensitive and precise analysis of sex-specific gene expression. Small regions of tissue that produce male sex combs and homologous female tissues will be isolated by laser capture microdissection, and RNA will be extracted from male and female samples comprising only ~100 cells. Quantitative transcriptome profiling will then be carried out using high-throughput, short-read sequencing, and the data will be assembled and analyzed with the help of the newly available genome sequences of several model species. The advantage of this approach is that the samples isolated by laser capture microdissection will be composed only of the cells that differentiate in a sex-specific manner, greatly increasing the power to detect gene expression differences between male and female tissues. This powerful yet technically demanding approach will be assisted by the acquisition of modern instrumentation and hiring of additional personnel.
Project Description: Project activities are just now beginning.
Jobs Summary: Research staff. (Total jobs reported: 0)
Project Status: Less Than 50% Completed
This award's data was last updated on Aug. 28, 2009. Help expand these official descriptions using the wiki below.