Grant: $244,324 - Department of Health and Human Services - Sep. 30, 2009
No votes have been cast for this award yet
Award Description: Understanding the Bacterial Ribosome assembly reaction is key to identifying potential new targets for the development of antibiotics. Molecular recognition by RNA is a key component of regulatory networks in living cells and therefore requires detailed biophysical characterization. Our models dissect energetically and kinetically these assembly reactions revealing their key molecular components. The purpose of this supplement is to obtain additional funding to hire a post-doc who has extensive experience running time-resolved hydroxyl radical footprinting experiments to setup and run our Kintek rapid flow mixer. We propose initially studying the Guanosine Riboswitch for the development of the time-resolved protocols. This system is multi-molecular, like the Ribosome, but binds Guanosine, which we can purchase. The system will allow us to rapidly put in place protocols for carrying out time-resolved experiments, while also providing an additional data set for methodological validation. Furthermore, the Riboswitch is in and of itself biologically significant, controlling major metabolic pathways in Bacteria. An additional post-doc in the lab will significantly accelerate the tempo of our research. Specifically, this will allow us to carry out time-resolved experiments immediately, in parallel with our production of Ribosomal proteins. This will result in an effective doubling of the pace of research in the lab, as we expect to begin collecting data on the Ribosome in late 2009, which we originally planned on starting only in 2011. Furthermore, we will broaden the biological scope of the research to include Riboswitch folding, opening up potentially new opportunities for independent investigation
Project Description: Not yet started
Jobs Summary: None to report (Total jobs reported: 0)
Project Status: Not Started
This award's data was last updated on Sep. 30, 2009. Help expand these official descriptions using the wiki below.