Grant: $175,392 - National Institutes of Health - Jul. 14, 2009
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Award Description: The molecular events that lead to proper patterning and reinnervation of the cornea following disease or injury remain largely unknown. The goal of our proposed research is to investigate keratocyte derived guidance cues to the regenerating axons during corneal reinnervation. The main purpose of the supplement award is to provide funds for research development support to enhance the scope and pace of Dr. Jain's scientific research and provide immediate economic stimulus as enisioned in the Recovery Act (ARRA).
Project Description: Laboratory diagnostic equipment (Li-Cor and Stereofluorescence microscope) were purchased to help with quantitative analysis of corneal nerve regeneration. Other supplies ordered were - diamond blades for creating lamellar dissection for murine model of corneal nerve regeneration. We have performed lamellar corneal flaps in murine cornea. The flap, bed and surrounding cornea were immunostained with antibodies (2H3, GAP-43, CGRP, EphB1, ephrinb1, NGF, BDNF) at time 0 and 1 week. The tissues have been processed for Western blot analyses and RT-PCR. Whole mount corneas have been examined by the newly purchased Stereofluorescence microscope. Mice homozygous for Thy-1 YFP have been ordered and once we obtain them we will establish a colony of homozygous Thy1-YFP mice for in vivo sterofluorescence examination.
Jobs Summary: One full-time job was created. This employment is a new-hire for UIC. Cecile Sato, PhD started employment on September 1, 2009. She is providing assistance with molecular techniques (immunofluorescence, Western blot analysis and RT-PCR) as well as establishing a colony of homozygous Thy1-YFP mice. (Total jobs reported: 1)
Project Status: Less Than 50% Completed
This award's data was last updated on Jul. 14, 2009. Help expand these official descriptions using the wiki below.