Grant: $49,903 - National Institutes of Health - Aug. 24, 2009
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Award Description: This supplement to grant number 01 DK 074565-01, A Cell Biological Approach to Lipid Absorption, is designed to speed the discovery of new information related to the control of dietary lipid absorption. As is well known, obesity is a current crisis of health care in the United States. The aim of the funded grant and the current supplement is to discover regulatory mechanisms in the complex process of dietary fat absorption. We have previously shown that the rate-limiting step in the absorption of dietary fat is its exit from the endoplasmic reticulum (ER) as triacylglycerol (TAG), a component of the intestine's unique lipoprotein, the chylomicron. We have reported that this exit step is performed by the formation of the pre-chylomicron transport vesicle (PCTV), a transport vesicle that takes the chylomicron to the Golgi. We plan to extend Specific Aim 1 of the funded grant by first establishing the central role of apolipoproteinB48 (apoB48) in the formation of the budding complex that is required to first select the cargo for inclusion in PCTV, next to deform the ER membrane and, finally, to cut it from the membrane so that it may travel to the Golgi. We plan to test the hypothesis that the phosphorylation of the small valosin interactive peptide (SVIP), whose phosphorylation we have identified as required for PCTV generation enables SVIP to interact with apoB48 and that in the absence of phosphorylation, there is no interaction of SVIP with apoB48. The second extension of the approved grant is to determine if the cytosolic extension of apoB48 is the part of apoB48 that interacts with SVIP. Since SVIP is a cytosolic protein, it would make sense that if interaction with apoB48 were to occur, a cytosolic component of apoB48 would be required. In this way apoB48, by extending into the sytosol, could signal to the developing PCTV that the chylomicron of which it is a component, is mature and ready to be included in the transport vesicle. Two first year Medical Students have selected aspects of this project to work on this summer funded by an NIH sponsored M1 Student Research Grant. If their interest continues, they will be given the opportunity to continue their work in the laboratory.
Project Description: As described in Award Description field.
Jobs Summary: 00 (Total jobs reported: 0)
Project Status: Not Started
This award's data was last updated on Aug. 24, 2009. Help expand these official descriptions using the wiki below.