Grant: $57,290 - National Institutes of Health - Sep. 30, 2009
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Award Description: In response to RFA Notice # NOT-OD-09-056, an administrative supplement is being requested to accelerate research studies proposed in NIH/NIGMS funded R01 GM081054 titled 'Norepinephrine Transport Regulation By Phosphorylation.' The major goal of the parent grant is to test the hypothesis that neurokinin 1 receptor/Protein Kinase C (NK1 RlPKC) -mediated norepinephrine (NE) transporter (NET) phosphorylation regulates NE transport. The aim of the proposed supplement is to facilitate two specific areas within the scope of GM081054 in achieving the goals of the parent grant. 1) Generate phospho-site NET mutants and determine the role of NET phosphorylation in NE transport modulation, and 2) Identify endogenous mediators of NE transport regulation in native placental trophoblasts. The proposed studies under the supplement will test two hypotheses; 1) that transporter phosphorylation and/or mutation of specific phospho-site/s on NET modulate NE transport function via altered ligand binding kinetics, iondependency, and influx/efflux ratios, and 2) that endogenous mediators linked to neurokinins regulate native NET phosphorylation. Thus, the studies addressing the influence of NET phosphorylation on NE transport properties and endogenous signals involved in native NET regulation will enhance and complement the progress made in the past two years. Successful completion of the studies proposed will bring insights to our understanding of native NET regulation and aid in gaining knowledge that could be of use in developing therapeutic strategies for the modulation of endogenous signals involved in NET regulation and for the reversal of compromised NET function seen in cardiovascular diseases and brain disorders.
Project Description: As defined in the Award Description field.
Infrastructure Description: N/A
Jobs Summary: None. (Total jobs reported: 0)
Project Status: Not Started
This award's data was last updated on Sep. 30, 2009. Help expand these official descriptions using the wiki below.