Grant: $218,661 - National Institutes of Health - Sep. 30, 2009
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Award Description: The long term goal of the original project is to understand how the functions of nuclear transport factors and histone chaperones are coordinated, and regulate the assembly of histones into specific chromatin domains. This project focuses on the role of histone chaperones in histone import and export, and the significant question of whether histone chaperones regulate the movement of histones in the cell. We have made good progress in the project and have published several papers. However some of the experiments described in the peer reviewed Aims of the original grant are proving more challenging and slower to complete than anticipated. I am requesting an Administrative Supplement to allow me to hire an advanced Ph.D level Research Associate, therefore creating a new job. This person will make significant contributions to the peer reviewed Aims 1 and 3 of this proposal, and will accelerate the tempo of scientific research. The Research Associate will already have the skill set to rapidly set up the biochemical and microscope based assays discussed in Aim 1, as well as perform the multiple chromatin immunoprecipitation experiments described in both Aims 1 and 3. This individual will allow us to quicken the pace of our research and enhance the value of the project by strengthening our findings through additional experiments. In addition I am requesting funds to purchase new equipment. This will increase the pace of our progress by relieving a bottle-neck in our ability to generate and test histone mutants and in our analysis of histone chaperones. We will also be able to buy equipment that is integral to the proposal, but is more than ten years old and will soon need replacing. Supplemental funds will allow us to increase the tempo of our research, attack some of the more challenging experiments in a more efficient manner and complete the proposed work. RELEVANCE: The correct assembly of nucleosomes is critical for maintaining genomic stability in all cells. The dysfunction of proteins involved in nucleosome assembly and remodeling pathways is also coincident with human disease and cancer. Supplemental funding will allow us to continue and complete our proposed study of this pathway.
Project Description: See Award Description;
Jobs Summary: None (Total jobs reported: 0)
Project Status: Not Started
This award's data was last updated on Sep. 30, 2009. Help expand these official descriptions using the wiki below.