Grant: $234,053 - National Institutes of Health - Sep. 30, 2009
No votes have been cast for this award yet
Award Description: This is a supplemental request to the parent grant, Biochemistry and Regulation of Cadherin Activity, that will not add to the scope or specific aims. Rather it will provide funds for the retention of a key person who was listed without salary on the original parent grant application to enable him to perform the project as originally proposed, and additional funds for supplies and equipment to accelerate the tempo of the research that was described in the original aims. Salary support is requested for retention of a current Research Associate/Postdoctoral Fellow in the laboratory who did not obtain the postdoctoral fellowships we had hope for. He will investigate the mechanisms of C-cadherin and E-cadherin regulation by protocadherins and growth factor signaling pathways in aims Band D. Additional supplies and other expenses are requested to accelerate the tempo of the research described in aim C. of the parent grant, which includes several supply and core facility intensive research efforts. These include the production of numerous different monoclonal antibodies in large amounts to make Fab fragments and the purification of large amounts of soluble E-cadherin and C-cadherin proteins from large scale cell culture in order to determine the crystal structures of Fab-cadherin complexes; the generation of additional conformation selective monoclonal antibodies; the analysis of large numbers of peptide samples by mass spectrometry for fine epitope mapping; and the purchase of numerous synthetic peptides to perform fine epitope mapping. Two pieces of equipment are requested to greatly facilitate our research efforts and accelerate the tempo of our research activities. A TIRF microscope will be used to analyze the organization of cadherins and associated cytoskeletal proteins in the plane of the membrane at high resolution during regulation of adhesion and cadherin activity states, and also for more routine fluorescence microscopy throughout many parts of the project. A versatile gradient HPLC/FPLC system will be used for the large amount of protein and peptide purification and analysis needed for the crystallization of Fab-cadherin complexes and fine epitope mapping experiments. RELEVANCE: Understanding the way that cadherin protein molecules are switched onloff at the surface of the cell to control their adhesive functions in tissues and developing methods to detect and control the functional state of cadherins on the cell has the potential to be used as diagnostic or therapeutic approaches for the treatment of many diseases, including cancer.
Project Description: See Award Description;
Jobs Summary: None (Total jobs reported: 0)
Project Status: Not Started
This award's data was last updated on Sep. 30, 2009. Help expand these official descriptions using the wiki below.