Grant: $100,000 - National Institutes of Health - Aug. 10, 2009
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Award Description: This administrative supplement award provides funding ($100,000) to purchase a new research microscope to facilitate and increase the rate of work on our studies of chromosome pairing in Drosophila. The work was previously approved under NIH grant number 5 R01 GM040489-18A2 funded from August 2008-July 2012. This project addresses the mechanisms that underlie chromosome segregation in meiosis. Meiosis is the specialized cell division that leads to production of gametes such as sperm and eggs and is an essential component of sexual reproduction. Errors in meiotic segregation lead to aneuploidy - wrong chromosome number - and account for a high proportion of genetic disease in humans. The proposed experiments utilize Drosophila melanogaster as a model system and focus on the functions of three newly identified meiotic proteins: Stromalin in Meiosis (SNM), Mod(mdg4) in Meiosis (MNM), and Sisters on the Loose (SOLO). SNM and MNM function together in male meiosis to provide stable connections between homologs. Experiments to learn about where SNM and MNM localize on chromosomes and how they interact with one another and with chromosomes to provide stable interhomolog linkages will be conducted. SOLO is expressed in both male and female meiotic cells and is required for cohesion between sister centromeres and formation of synaptonemal complexes, structures that help align homologs during recombination. Experiments to delineate the relationship between SOLO and the cohesin complex and to ascertain the role of SOLO in homolog pairing and synapsis will be carried out. Experiments to identify pairing sites on autosomes will also be conducted. The administrative supplement award (2 R01 GM040489-19S1) is for the period August 8, 2009 - July 31, 2010. It provides funds to purchase a Zeiss Axioobserver Z1 motorized, inverted fluorescence microscope with a high-resolution digital camera and software for imaging, optical sectioning and 3D rendering, and deconvolution. Fluorescent microscopy is central to nearly all of the experiments proposed in the main grant but is currently supported only by a single, 21?year?old microscope that lacks some of the capabilities needed for our studies, is only partially automated, is subject to unpredictable fluctuations in illumination intensity and filter alignment, has limited resolution and sensitivity, and is breaking down at growing frequencies. The new microscope will support live imaging, FRET and multi?channel imaging and will be fully automated with enhanced resolution and sensitivity, stable illumination, focus and alignment. The availability of a second, more powerful and efficient microscope system will greatly enhance the rate of progress in cytological studies and will expand the range of experiments that can be conducted.
Project Description: We have initiated the paperwork to order the microscope. The microscope is sold by only one vendor, Zeiss. As of this writing, a request for a sole-source requisition is awaiting approval in the University of Tennessee Purchasing Office. Once the order is placed, construction and shipping of the microscope is expected to take about 10 weeks. The university has agreed to carry out some renovations of my laboratory to provide additional microscopy space to house the new instrument. The UT Facilities department began work on that renovation on September 14, 2009. The renovation is expected to be completed by the end of November, 2009.
Infrastructure Description: This is a conditional field. This grant is NOT for Infrastructure projects.
Jobs Summary: No jobs will be created by this award. (Total jobs reported: 0)
Project Status: Less Than 50% Completed
This award's data was last updated on Aug. 10, 2009. Help expand these official descriptions using the wiki below.