BRIGHAM AND WOMEN'S HOSPITAL, INC., THE
Grant: $50,760 - National Institutes of Health - Jul. 20, 2009
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Award Description: The Supplementary Funding for my K08 through the Recovery Act has allowed me to hire a new technician in my laboratory. Peter Kelsey, a chemistry major from Wake-Forrest is a perfect addition to my research team. Given his chemistry background, he is well-suited for studying the interaction between prostaglandin and wnt signaling. He has started last month and is still being trained in the major techniques in the laboratory, but has already begun to conduct his own experiments. Furthermore, part of the funding has been dedicated to purchase a new zebrafish tank system to increase the speed at which we can do our experiments. This system, ordered from Aquaneering, Inc. in San Diego, will arrive later this fall.
Project Description: The aim of the Administrative Supplement of this award was to further investigate the role of prostaglandin and wnt signaling during zebrafish liver development. This has a great impact of our understanding of liver disease in humans, particularly for liver regeneration and cancer formation. Work on the stated Aims of the Supplementary Funding Application has begun immediately. We are currently delineating the interaction of prostaglandin and wnt signaling in liver development. Our experiments are progressing well, as we have been able to use fluorescent reporter fish to co-localize wnt activity to the liver, and noted an increase in wnt signaling in response to prostaglandin exposure. Furthermore, we are optimizing the technique to harvest fluorescently labeled liver cells from the zebrafish by FACS, to submit these to qPCR and microarray analysis in the future. Similarly, we have begun to analyze cell cycle and proliferation in the liver in response to prostaglandin and wnt signaling. I expect that these experiments will result in a publication in a respected journal, and that the project can be completed within the anticipated time frame.
Project Status: Not Started
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