Grant: $9,502 - National Institutes of Health - Jul. 14, 2009
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Award Description: The parent R01 is aimed at elucidation of molecular mechanisms underlying axon pathfinding focused on the process of intraretinal axon projection by retinal ganglion cell (RGC) axons. The summer research project is aimed at establishment of a protocol of RGCs as dissociated cell culture and use the culture to study signaling mechanisms invovled in axon growth and guidance. A student from one of our collaborators' group at Worcester Polytechnic Institute will carry out this summer project. Majored in chemical engineering, the student is interested in a career in biomaterial and bioengineering . She will learn to culture primary neurons and neuronal cell lines, carry out time-lapse microscopy experiments and data analysis. This summer research project will provide useful hands-on experience and technical training relevent to her undergraduate thesis research in the fall semester which is to examine neurite/axon growth on various polymer materials, and to encourage her to pursue a career in health-related sciences.
Project Description: Various protocols for culturing dissociated primary RGC cells were tested. Individual RGC cells were present in the culture, which account for about 5-10% of the total cells. The summer student intern additionally worked out a protocol for culturing RGC-5 cells, a retinal ganglion cell line. By using primary RGC axon cultures and RGC-5 cells, the student tested the effects of a group of small molecules on the growth and morphology of the neurites. Neurite lengths of the RGC-5 cells and the growth cone sizes of the RGC axons were determined before and after the addition of small molecules. Her preliminary results indicate that it is feasible to screen for factors that promote axonal growth and regeneration by using a combination of these two culture models.
Jobs Summary: N/A (Total jobs reported: 0)
Project Status: More than 50% Completed
This award's data was last updated on Jul. 14, 2009. Help expand these official descriptions using the wiki below.