Grant: $237,695 - National Institutes of Health - Jul. 16, 2009
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Award Description: In this Administrative Supplement, we will examine the potential role of PECAM1 in fluid shear stress-mediated VEGFR2/Gab1/eNOS signal pathway, which is involved in flow atheroprective effects.
Project Description: We prepared the reagents and set up shear stress experiments. We isolated endthelial cells from human umbilical vein and cultured these cells. We transfected the cells with PECAM1 small interference RNA (siRNA) and exmined the expression of PECAM1 in cell lysates by immunoblotting with anti-PECAM1 antibody. We found that siRNA efficiently knockeddown PECAM1 in endothelial cells. We will perform shear stress experiments on siRNA-transfected cells and examine the effect of PECAM1 downregulation on shear stress-mediated Akt and eNOS activation in endothelial cells.
Jobs Summary: The University of Rochester created/retained an Postdoctoral Research Associate. (Total jobs reported: 1)
Project Status: Less Than 50% Completed
This award's data was last updated on Jul. 16, 2009. Help expand these official descriptions using the wiki below.