Grant: $17,610 - National Institutes of Health - Jun. 4, 2009
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Award Description: This is an administrative supplement to provide a summer research experience 'Mitochondrial Ca2+ Transport in Heart Cells . Our original application proposed to investigate the molecular and biophysical characteristics of the mouse mRyR. In the studies described in this supplement, our summer student will investigate the role of mRyR in regulating mitochondrial membrane potential, calcium entry, and reactive oxygen species generation using primary cultures of cardiac myocytes from newborn control or RyR knock-out mice. The student will learn state-of-the-art molecular and cellular biological techniques in a laboratory that has a history of mentoring undergraduate students and guiding them into a career in health related sciences.
Project Description: These proposed studies are relatively limited, and thus should be easily performed over the course of two summers by a well-trained undergraduate student. This student will be recruited via a number of potential programs that the laboratory has available, and he or she will be closely mentored by the PI and his collaborators and will be trained by experts in each of these techniques. Therefore, we believe that these studies will greatly enhance the information we have generated on the role of calcium regulation by RyR1 in the mitochondria of the mammalian heart, while, at the same time, provide an excellent environment for a student to further his or her research experience. A junior undergraduate student at the University of Rochester was hired this summer to work on this project. The project focused on the characterization of the role of mitochondrial permeability transition pores in regulating mitochondrial Ca2+ homeostasis and reactive oxygen species generation. The student has learned how to isolated mitochondria from rat and mouse heart. They performed several assays to determine mitochondrial electron transport chain activities, mitochondrial Ca2+ uptakes, and mitochondrial membrane potentials. The results show that inhibitors for mitochondrial fission process also inhibit Complex I and V activities.
Jobs Summary: The University of Rochester created/retained a Student. (Total jobs reported: 0)
Project Status: Less Than 50% Completed
This award's data was last updated on Jun. 4, 2009. Help expand these official descriptions using the wiki below.