Grant: $474,750 - National Institutes of Health - May. 14, 2009
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Award Description: This project is to define the defect in T cell positive selection Themis (previously known as MG143) knockout mice in terms of signaling pathways that are affected, the interactions with other members of the TCR-signaling cascade, and changes in gene expression. We have shown that there are altrations in calcium and Map kinase signaling pathways. These will be investigated further. Bone marrow stem cells from Themis mice will be transduced with the Themis gene or mutated versions of it, and used to reconstitute lethally irradiated mice. This will enable us to understand the structure-function or regions of Themis. We will test the function and importance of the nuclear localization sequence, the potential site for phosphorylation by ATM, the polyproline (SH3-binding) region, a region for binding pleckstrin homology (PH) domains, and potential tyrosine phosphorylation sites. We will envestigate interactins with other proteins using FRET mocroscopy and biomolecular fluorescence complementation.
Project Description: As defined in Award Description field.
Jobs Summary: We were able to hire a Research Technician as a result of this funding. (Total jobs reported: 3)
Project Status: Less Than 50% Completed
This award's data was last updated on May. 14, 2009. Help expand these official descriptions using the wiki below.