Grant: $21,899 - National Institutes of Health - Jun. 1, 2009
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Award Description: This proposal is based on our preliminary data supported by the parent grant, in which we utilized the exquisite sensitivity of multidimensional protein identification technology (mudPIT) and an inducible monocyte progenitor cell line (Hoxa9-ER4) to identify a novel differential-induced integral membrane plasminogen receptor. PIgoRkt (C9orf46 homolog). We have recently found that this novel protein markedly stimulates cell surface plasminogen activation. This structurally unique plasminogen receptor,thus, represents a novel control point for regulating cell surface proteolysis. In addition, our laboratory has demonstrated that the interaction of plasminogen with monocytoid cells inhibits apoptosis and that this requires the proteolytic activity of plasmin on the cell surface. The objective of this proposal is to test the role of Plg-Rkt<T in protection from monocyte/macrophage apoptosis. To address our hypothesis our specific aim for this summer is: 1) to test the role of Plg-Rkt in plasminogen-dependenl inhibition of apoptosis. Expected Outcome if our hypothesis is supported, we expect that anti-PIg-Rkt will neutralize the anti.-apoptotic effects of plasminogen so that the number of non-viable cells will be increased, internuc!eosomal DNA fragmentation will be increased, and active caspases 3, 8 and 9 will be increased relatlive to cells treated with isotype control
Project Description: As defined in Award Description field.
Jobs Summary: Two paid summer scientlfic internships to students were supported with this supplement. In addition, small supplements were paid to three volunteer high school scientific interns. (Total jobs reported: 1)
Project Status: Less Than 50% Completed
This award's data was last updated on Jun. 1, 2009. Help expand these official descriptions using the wiki below.