Grant: $38,577 - National Institutes of Health - Jun. 2, 2009
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Award Description: Identification of All Functional Elements in Selected Model Organism Genomics
Project Description: We have developed a method to automatically select large quantities of a specific genotype of Caenorhabditis elegans animals from a mixed pool. Using GFP-marked balancer chromosomes and laFACS (live animal Fluorescence Activated Cell Sorting), high-purity selection of over 100,000 animals of a defined genotype can be achieved in approximately one hour of sort time. While this method has broad applicability, we demonstrate its utility here for a case where high accuracy and continued development of the sorted animals is critical. Automated sorting is combined with systematic RNAi to identify genetic modifiers of the maternal-effect lethal mel-28 mutation. MEL-28 is a key regulator of nuclear function in the early embryo and, fittingly, the screen results uncover components of the nuclear envelope and chromatin organizers, demonstrating the power of this approach.
Infrastructure Description: N/A
Jobs Summary: None (Total jobs reported: 0)
Project Status: More than 50% Completed
This award's data was last updated on Jun. 2, 2009. Help expand these official descriptions using the wiki below.