UNIVERSITY OF ALABAMA AT BIRMINGHAM
Grant: $251,908 - National Institutes of Health - Sep. 24, 2009
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Award Description: High density lipoprotein (HDL) and its major protein component apolipoprotein (apo) A-1 exert prominent anti-inflammatory and anti-oxident effects. Preliminary data presented in the parent application show that the apoA-1 mimetic peptide 4F, whose structure is based on the helical repeating domains of apoA-1, promotes the differentiation of monocytes to DCs and thus enhances coupling of innate and adaptive immune responses. Monocytes and macrophages may both undergo differentiation to form DCs. Studies are designed to assess effects of 4F on phenotypic and functional changes in these cell types. Preliminary data suggest that 4F induces the differentiation of an anti-inflammatory type of macrophage. There are many subsets of macrophages with anti-inflammatory properties. The 4F/apoA-1 modified macrophages adopt a phenotype that reduces cellular injury and facilitate repair mechanisms at sites of inflammation. This supplement is to identify the subset insuced by 4F and/or apo A-1 by carrying out transcription profiling and obtaining gene signatures of the cells by Affymetrix. These results will be further validated by other cell surface marke and cytokine panels.
Project Description: High density lipoprotein (HDL) and its major protein component apolipoprotein (apo) A-1 exert prominent anti-inflammatory and anti-oxident effects. Preliminary data presented in the parent application show that the apoA-1 mimetic peptide 4F, whose structure is based on the helical repeating domains of apoA-1, promotes the differentiation of monocytes to DCs and thus enhances coupling of innate and adaptive immune responses. Monocytes and macrophages may both undergo differentiation to form DCs. Studies are designed to assess effects of 4F on phenotypic and functional changes in these cell types. Preliminary data suggest that 4F induces the differentiation of an anti-inflammatory type of macrophage. There are many subsets of macrophages with anti-inflammatory properties. The 4F/apoA-1 modified macrophages adopt a phenotype that reduces cellular injury and facilitate repair mechanisms at sites of inflammation. This supplement is to identify the subset insuced by 4F and/or apo A-1 by carrying out transcription profiling and obtaining gene signatures of the cells by Affymetrix. These results will be further validated by other cell surface marke and cytokine panels.
Jobs Summary: In process of creating a new reseearch staff position (Total jobs reported: 0)
Project Status: Not Started
This award's data was last updated on Sep. 24, 2009. Help expand these official descriptions using the wiki below.
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