Grant: $217,379 - National Institutes of Health - Sep. 9, 2009
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Award Description: The primary objective of the administrative supplement is to hire a postdoctoral research associate who will perform proteomics and confocal microscopy studies to identify the protein kinase A ancoring proteins (AKAP) that is necessary for the nuclear accumulation of activated p90 ribosomal S6 kinase 1 (RSK1). These studies are based on the findings that form the foundation of the parent grant that inactive RSK1 associates with the regulatory subunit (PKAR) of type I cAMP-dependent protein kinase (PKA) while the active RSK 1 binds the catalytic subunit (PKAc) of PKA. By its association with PKA subunits, the RSK1 ensures its association with AKPs. The PKA subunit-medicated association of RSK1 with AKAPs is necessary for the active RSK1 localization and accumulation in the nuclear. However, the identify of the AKAP(s) that RSK1 associates with or the AKAP9(s) required for its nuclear localization remain unknown. Preliminary data show that by immunoprecipiting RSK1 from cells treated with peptides Ht31 dissociates AKAPs from the PKA/RSK1 complex, the presence of certain proteins in the complex is decreased. Therefore, the research objectives of the postdoctoral research associate to be hired will be to perform proteomics analysis by LS/MS/MS to identify these proteins which will include the AKAPs that bind the PKA/RSK1 complex. Thereafter, the postdoctoral research associate will individually silence the expression (by siRNA) approaches) of each of the identified AKAps to determine which of these AKAps alters the nuclear accumulation of active RSK1. These studies are within the scope of the parent grant and do not alter the direction of our project. The novel information gleaned will increase the pace as well as the tempo of our research. Moreover, they will ensure the renewal of the parnet grant which will help retain the research personnel in their positions or permit the hiring of new people. These goals are consistent with the missions of the American Recovery and Reinvestment Act.
Project Description: Project not started.
Jobs Summary: As the project has not yet started, there are no jobs created or retained. (Total jobs reported: 0)
Project Status: Not Started
This award's data was last updated on Sep. 9, 2009. Help expand these official descriptions using the wiki below.