Grant: $13,185 - National Institutes of Health - Jun. 2, 2009
33% voted satisfied - 67% voted not satisfied - 3 vote(s) cast
Award Description: The parent greant (R01 DE016059) was awarded by NICDR/NIH for research on the 'Sex Steroids and TMJ Pain'. This supplement provides short-term research experience for two college graduates during the summer preceding their entry into the first year of the DDS program at Baylor College of Dentistry. The purpose of the supplement is to: 1. recruit promising individuals of exceptional aptitude with a basic skills set to advance the research goals of the parent grant, 2. provide a source of income for pre-dentistry residents during this difficult economic climate, 3. give the students the opportunity to engage in a clinically relevant research project that can be completed in an 8-10 week period. This opportunity will provide the students with training in new techniques and will lead to the collectio of original data, which can be used for presentations to be given at the 2009 Hinman Student Research Symposium and the 2010 AADR annual meeting, and 4. introduce the students into the basic and clinical research resources available at Baylor College of Dentistry, with the hope that in doing so, interest in pursuing a career in dental academic research will be generated.
Project Description: Student 1; Objective: Intra-articular administration of pain releaving and antibiotics drugs have been used to reduce TMJ pain. Longer drug-release profiles of these drugs would reduce pain for greater periods of time; injecting gelatin micro-capsules into the joint might extend pain relief. Methods: We injected control or drug filled micro-capsules into the TMJ, with and without Complete Freund’s adjuvant (CFA), of rats or in vitro. Pain response (i.e., meal duration) was measured, and the rats killed 0, 5 and 10 days after injection. Micro-capsules and cytokines were quantified in the joint using histology and ELISA. Results: The micro-capsules did not degrade in vitro. Conclusions: Injection of the joint with micro-capsules did not exacerbate the pain response and degraded slowly over a 10-day period suggesting that gelatin micro-capsules are a viable mechanism for long-term drug release in patients with an arthritic TMJ. Moreover, inflammation resulted in micro-capsule degradation in vivo but not in vitro suggesting degradation of the beads was dependent on inflammatory mediators such as gelatin metalloproteases MMP2/9. Student 2; Objective: Complement and its product, C5a, influence the development of inflammatory disease; C5a can also contribute to neuronal responses during inflammation, i.e., allodynia and hyperalgesia. We evaluated C5a and it receptor (R)-expression in the trigeminal ganglia (TG) and TMJ after TMJ inflammation. Methods: CFA was injected into the TMJ male rats. Joint tissue and TG were collected after 24 or 48 hr for Western blot analysis or processed for immunohistochemical staining. Results: C5aR protein was constitutively expressed in both the TMJ and TG with prominent staining of blood vessel endothelial cells in the TMJ. Upregulation of C5a protein occurred in the TMJ after CFA injection. Conclusions: The results show TMJ C5a and its R are invovled in TMJ inflammation by the recruitment of leukocytes and macrophages.
Jobs Summary: These jobs that were created were summer research positions for dental students that were either recently accepted by the College or were students that had completed their first year of dental school. (Total jobs reported: 2)
Project Status: Completed
This award's data was last updated on Jun. 2, 2009. Help expand these official descriptions using the wiki below.