Grant: $444,960 - Department of Health and Human Services - Sep. 11, 2009
0% voted satisfied - 100% voted not satisfied - 1 vote(s) cast
Award Description: The viral hemorrhagic fevers (VHFs) pose a threat as possible bioterrorist agents as well as to populations in sub-Saharan Africa where many VHFs are endemic. The non-specific clinical presentation of the VHFs makes them extremely difficult to diagnose clinically and no commercial diagnostic kits exist. The primary objective of this project is to establish rapid and sensitive multiplex molecular assays for the VHFs, as well as for other diseases included in their differential diagnosis, and to validate the assays using clinical samples collected from West Africa in the course of other research projects. Features that will enhance the probability of success of this proposal include access to the clinical specimens from West Africa where numerous VHFs are endemic; the expertise of the investigators in clinical medicine, microbiology, bioinformatics, and molecular diagnostics; a novel technology for mass spectrometric (MS) detection of PCR amplification products; a sensitive pathogen detection microarray platform (GreeneChips); and banks of primer sequences, cDNA targets and mass tags. The specific aim of the proposal is to develop a multiplex MassTag PCR assay for African VHFs, as well as for an array of additional pathogens included in their differential diagnosis.
Project Description: The award for this project was made just ~3 weeks ago (September 12, 2009) so the activities and goals for this quarter are oriented toward establishing the organization and infrastructure to implement the project, including: 1) Execute a sub-contract with our collaborators at Columbia University, 2) Hire a laboratory technician at Tulane, 3) Procure the necessary equipment, including thermocycler, mass spectrometer, nitrogen generator, and consumable supplies, 4) Hold a organizational and training meeting with Columbia University, and 5) Identify the 10 organisms and primer sets to be included in the initial rendition of the MassTag PCR assay and begin generating the template standards.
Jobs Summary: None (Total jobs reported: 0)
Project Status: Less Than 50% Completed
This award's data was last updated on Sep. 11, 2009. Help expand these official descriptions using the wiki below.