Grant: $49,680 - National Institutes of Health - Aug. 24, 2009
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Award Description: Based on my K08 hypothesis that selective combinations of adhesion molecule expression identifies subsets of T cells that preferentially home to the colon and results from the K08 aims, we identified not one but two candidate chemokine receptor genes that are highly up-regulated in the colon as opposed to the small intestinal T cells. Preliminary results from these studies suggest that these molecules may play an important role in selective colon T cell homing under homeostatic or normal physiologic conditions. For this supplement I hypothesis that the identified candidate molecules, CCR1 and GPR15, are also important targets in selective mechanism for the recruitment of T cells from the blood to the inflamed colon. Based on this hypothesis, the specific aims of this supplement proposal are: Aim 1. Determine role of CCR1 in T cell trafficking to the inflamed colon. Here we will study the role of CCR1 by performing adoptive cell transfers in mice with colitis under competitive condition using CCR1 +/+ and CCR1 -/- donor T cells in short-term homing assays. We will determine differential trafficking between CCR1+/+ and CCR1 -/- T cells to the colon (in comparison with other tissues such as the small intestine) using immunohistochemistry and flow cytometry by staining for congenic markers. Aim 2. Determine role of GPR15 in T cell trafficking to the inflamed colon. Here we will study the role of GPR15, using GPR15 +/- GFP knock-in mice and using similar studies described in aim1. We will be able to identify GPR15+/+ and GPR15 -/- T cells by their absence and presence of GFP respectively. Identification of not only one but two trafficking receptors as targets for colon T cell trafficking is exciting but also challenging to complete within the K08 term under homeostatic and inflammatory conditions. The research proposed here is within the scope of the parent grant or my K08. Supplement support not only will accelerate the pace of the research described but will allow me to keep and increase hours of an experienced and capable part-time research assistant
Project Description: As defined in the Award Description field
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Project Status: Less Than 50% Completed
This award's data was last updated on Aug. 24, 2009. Help expand these official descriptions using the wiki below.