Grant: $69,362 - National Institutes of Health - Sep. 25, 2009
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Award Description: The long-term goal of the current project (2 R15 GM069562-02) is to develop a fundamental understanding of the regulatory control of terpene production in Artemisia annua so that in planta metabolic manipulation of terpenoid biosynthesis can eventually be developed not only for artemisinin (AN), but also other therapeutically important plant produced terpenoid drugs. The current work has 3 objectives: 1) To compare the relative expression of the early (DXS, DXR, HMGR, and FPS) and late (ADS and CYP71AV1) genes in terpenoid biosynthesis with levels of both AA and AN production in A. annua seedlings as they are shifted from vegetative growth to conditions that induce flowering. With supplemental funds we proposed doing the following that are encompassed within the original IRG-approved scope as part of our original Objective #1 (see above): 1. Use LC/MS to compare AN and AN metabolites (artemisinic acid, dihydroartemisinic acid and AN) production in shoots that begin to develop roots in vegetative plants. This is directly linked to Objective # 1 because it enables us to compare unrooted shoots to rooted shoots during the vegetative stage of growth. This was not possible until little over a year ago when we obtained a reliable, high AN producing clone of A. annua that can be cultured in vitro as either shoots or as rooted shoots. We have been culturing this clone for over a year and using it in other experiments aimed at overproduction of AN, but not related to a fundamental study of developmental regulation. 2. We will further analyze the transcript levels of the genes in the AN biosynthetic pathway (identified in the original IRG-approved scope) and establish correlations between those genes that may get turned on or off at the level of transcription as the roots develop during the vegetative growth stage and especially while the roots are being initiated. Together these additional data will enable us to better correlate artemisinin biosynthesis with the development of roots during the vegetative developmental phase of A. annua plant growth thereby better enabling completion of our original Aims.
Project Description: Due to the award start date of 09/30/09, there are no quarterly activites this quarter.
Jobs Summary: N/A (Total jobs reported: 0)
Project Status: Not Started
This award's data was last updated on Sep. 25, 2009. Help expand these official descriptions using the wiki below.